Authors
Mihail Sarov
Summary
Before you start with the protocol, design the recombineering primers. They should include 50bp homology arms targeting the tagging site and 21 first bases of the tag region to amplify. Please take care of orientation of homology arms. All volumes are PER WELL of a 96-well plate.Further details
The protocol was published on Protocol Exchange on 27 May 2009. To see the entire protocol, click on the source link.Advertisement
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