Authors
Winfried Wiegraebe, Yucai Xie, Tong Yin, Danny Stark, Katherine Perko, Richard Alexander, Joel Schwartz, Xi He, and Linheng Li
Summary
This protocol describes the use of two-photon microscopy to image the dynamic behavior of hematopoietic stem cells interacting with their niche. To distinguish the eGFP expressing cells from auto-fluorescent background, we use spectral finger-printing. We include image processing steps to visualize the results and extract quantitative information.Further details
The protocol was published on Protocol Exchange on 19 February 2009. To see the entire protocol, click on the source link.Advertisement
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