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Cloning circadian promoters

External protocol Created on 30 Apr 2014

Authors

Eun-Deok Kim and Z. Jeffrey Chen

Summary

Regulation of circadian clock genes is difficult because their expression levels alter during day and night. To control expression of CCA1 that is repressed during the day and upregulated at night1 we overexpress CCA1 or cca1-RNAi using the TOC12 promoter that drives the expression during the day.

Further details

The protocol was published on Protocol Exchange on 8 January 2009. To see the entire protocol, click on the source link.

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