Authors
Erika Lackey and Z. Jeffrey Chen
Summary
The electrophoretic mobility shift assay (EMSA), also known as “gel shift assay”, is used to examine the binding parameters and relative affinities of protein and DNA interactions. We produced recombinant CCA1 protein and tested its binding affinity for the promoter fragments that contain CBS (AAAAATCT) or evening element (EE, AAAATATCT)1 using a modified procedure adopted from published protocols2,3.Further details
The protocol was published on Protocol Exchange on 8 January 2009. To see the entire protocol, click on the source link.Advertisement
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