Authors
Valentina Gandin, Pier Carlo Marchisio, and Stefano Biffo
Summary
This procedure describes a method for isolation of murine hepatocytes using a modified two-step perfusion method. This protocol allows to measure the level of protein synthesis in primary hepatocytes after stimulation with growth factor and hormones. The advantage of this procedure is to easily perform a 35S-metabolic labelling to measure the level of protein synthesis upon stimulation ex vivo.Further details
The protocol was published on Protocol Exchange in 2008. To see the entire protocol, click on the source link.Advertisement
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