Authors
Koji Atarashi and Kenya Honda
Summary
The lamina propria (LP) CD11c+ antigen-presenting cells directly sample the luminal contents and direct CD4+ T cells to differentiate into TH1, TH2 or TH17 cells (refs 1,2). Mouse CD11c+ LP cells consist of more than three subsets distinguished by different expression of cell surface markers, such as CD11b, CD70, CX3CR1, and CD103; and it has been reported that each subset has a distinct mission (refs 3-7). This protocol details a method to isolate LP CD11c+ cells with high yield, viability and purity (see also ref. 8).Further details
The protocol was published on Protocol Exchange in 2008. To see the entire protocol, click on the source link.Advertisement
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