Authors
Bruce E. Tabashnik, David W. Crowder, and Yves Carrière
Summary
Genetically modified crops producing insecticidal proteins from Bacillus thuringiensis (Bt) for insect control have been planted on more than 200 million ha worldwide since 1996 [1]. Evolution of resistance by insect pests threatens the continued success of Bt crops [2, 3]. To delay pest resistance, refuges of non-Bt crops are planted near Bt crops to allow survival of susceptible pests [4, 5]. We used computer simulations of a population genetic model to determine if predictions from the theory underlying the refuge strategy match outcomes in the field documented with monitoring data [6]. The computer program is called SERBt for Simulated Evolution of Resistance to Bt crops. For the six major pests modeled, the simulation results corresponded with the field data. In particular, the simulations indicated that resistance would evolve fastest in Helicoverpa zea, a major cotton pest in the U.S., and this insect was the first with documented field-evolved resistance to a Bt crop. The model can be readily modified to incorporate key biological parameters for many insects.Further details
The protocol was published on Protocol Exchange in 2008. To see the entire protocol, click on the source link.Advertisement
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