Authors
Kazushi Imai and Yasunori Okada
Summary
To detect activities of MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9, use radio-labeled fibrillar collagen (for MMP-1), gelatin (for MMP-2 and MMP-9) and carboxymethylated transferrin (for MMP-3 and MMP-7) substrates for the assays 1, although these can be substituted with commercially available FITC-labeled substrates and fluorogenic synthetic peptides for the MMPs. Zymography using gelatin or casein substrate gels is also useful to monitor gelatinolytic activity of MMP-2 and MMP-9 or caseinolytic activity of MMP-3 and MMP-7 in each column fraction, although zymography is not suitable for calculation of purification fold and recovery of proteinases.Further details
The protocol was published on Protocol Exchange in 2008. To see the entire protocol, click on the source link.Advertisement
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