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High-throughput Hisx6-tagged protein purification

External protocol Created on 03 May 2014

Authors

Chien-Sheng Chen and Heng Zhu

Summary

We have developed a high-throughput protein purification protocol that allows us to purify ~4,000 proteins within 10 hours from prepared culture. By combining steps of cell lysis and protein capture on affinity resins in sealed filter plates, we reduced the number of pipetting steps and thus, human errors.

Further details

The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.

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