Authors
Wenwei Hu, Zhaohui Feng, Angelika Teresky, and Arnold Levine
Summary
This protocol describes a procedure to search for novel p53 target genes. First, screen for potential p53 target gene using p53MH algorithm. Then, test these candidate genes using a series of molecular biological assays, including Chromatin immunoprecipitation assay, luciferase reporter activity assay, and regulation of the expression levels of mRNA and protein of these genes by p53 protein. This protocol also includes some assays to measure the mouse implantation and reproduction used in our study.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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