Authors
Naxin Jiang, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding
Summary
Azocoll, the Azo dye-impregnated collagen (Sigma) is used as a chromogenic non-specific substrate for protease activity assay. Upon proteolysis, soluble peptide fragments which is purple in color due to Azo dye-impregnation, are released and can be detected by absorbance at 550 nm.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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