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ELISA-based protein-PAMP interaction assay

External protocol Created on 03 May 2014

Authors

Naxin Jiang, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding

Summary

The interaction between HMC or metHb with LPS or LTA are examined by ELISA using LPS- or LTA-immobilized plates and probed with anti-hemocyanin or anti-hemoglobin antibody. To test the specificity of the ELISA, we further examined whether or not pre-incubation of HMC or Hb with LPS or LTA could reduce the ELISA-readout in a dose-dependent manner.

Further details

The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.

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