Authors
Naxin Jiang, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding
Summary
To determine the specificity of the microbial proteases in triggering the respiratory proteins to produce bactericidal ROS, we compared the level of bacterial clearance of protease-producing and non-producing strains of typical Gram-negative and Gram-positive bacteria. For Gram-negative bacteria, we chose Pseudomonas aeruginosa strain PAO-Iglewski which produces PAE (the major extracellular protease virulence factor), and the PAE-knockout mutant PAO-B1A1 which does not produce PAE1. For Gram-positive bacteria, we cultured Staphylococcus aureus PC1839 strain which produces active V8-protease, and AK3 strain which is a V8 protease-mutant2. The genes controlling PAE in P. aeruginosa and the V8-protease in S. aureus are under the control of quorum-sensing signals. Therefore the culture conditions which facilitate the production of active extracellular proteases are utilized.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 413
- Comments 0