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Measurement of phenoloxidase activity

External protocol Created on 03 May 2014

Authors

Naxin Jiang, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding

Summary

In the chelicerate, hemocyanin is the functional substitution of prophenoloxidase (PPO). Upon activation, hemocyanin is converted to phenoloxidase (PO), which oxidizes the phenolic substrate such as 4-methylcatechol to produce quinone product, which has a maximum absorbance at 405 nm.

Further details

The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.

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