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In vitro antimicrobial assay of metHb-mediated ROS production using mammalian RBC

External protocol Created on 03 May 2014

Authors

Naxin Jiang, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding

Summary

We hypothesized that upon systemic infection, the hemolysin-producing microbe lyses the erythrocyte and releases hemoglobin. Then microbial proteases and PAMPs enhance the ROS production by the metHb. Here, we used the mammalian (rabbit or human) red blood cell (RBC) to evaluate the antimicrobial activity of the metHb-mediated ROS production.

Further details

The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.

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