Authors
Naxin Jiang, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding
Summary
Here we describe the chemically-reconstituted system for evaluating the antimicrobial activity of the microbial protease-mediated ROS production from the hemocyanin-prophenoloxidase (HMC/PPO)or hemoglobin. The antimicrobial activity is examined by endpoint measurement of the remnant microbial population or by real-time observation of the bactericidal process. To demonstrate the specificity of PO-induced ROS-production which causes the antimicrobial activity, PTU is used here as a specific inhibitor of PO activity.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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