Authors
Naxin Jiang, Nguan Soon Tan, Bow Ho, and Jeak Ling Ding
Summary
This protocol describes the isolation and purification of hemocyanin from the horseshoe crab. In the open-circulating system of the horseshoe crab, hemocyanin is the most abundant extracellular protein, constituting >90% of the total proteins. Since hemocyanin exists as a 48-mer holo-protein (MW= 3,500 kDa)1, its sheer size makes it readily separated by gel-filtration chromatography from other major plasma proteins, such as the C-reactive protein which exists as a pentamer of 150 kDa, or α-2-macroglobulin which exists as a monomer of 180 kDa2,3.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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