Authors
Charles Egwuagu, Cheng-Rong Yu, Ahjoku Amadi-Obi, Xuebin Liu, Rashid Mahdi, and Yun Sang Lee
Summary
IL-27 is constitutively expressed in retina and inhibits T cells that mediate EAU. IL-27 is predominantly expressed in antigen-presenting lymphoid cells such as dendritic cells. Using a combination of RT-PCR, Western blot analyses we demonstrate expression of IL-27 in the retina and retina cells. Expression of IL-27 was localized to the retinal ganglion and photoreceptor cells as well as the retina pigmented epithelium (RPE) by immunohistochemical analysis. Expression of IL27 in the retina was found to be positively regulated in retinal cells by the proinflammatory cytokine, IFN-γ and chromosome immunoprecipitation assay (Chip) revealed direct binding of STAT1 transcription factor to the IL27 promoter.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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