Authors
Abhishek D. Garg, Dhananjay D. Garg, and Rajshekhar V. Hippargi
Summary
Human space technology is currently advancing with hasty pace. Top space agencies are now proposing their plans of establishing extra-terrestrial human life. With this background it’s vital to know exact effects of phenomenon like hypergravity, experienced during space flights on development of metazoan embryos. In this perspective it is very important to establish proper animal models for studying the effect of hypergravity on animal development. In the present protocol, an attempt has been made to establish the embryos of mollusc, Limnaea as model for studying the effect of hypergravity on cleavage timings of animal-embryos. Limnaea embryos/eggs are being considered suitable as models for this protocol because of several characters like, (1) Transparency of embryo, which allows easy visualization of developmental stages; (2) Limnaea embryos resemble human ones with respect to egg nature (isolecithal) and cleavage (holoblastic) and (3) Limnaea embryos show spiral cleavage, which is thermodynamically the most stable cleavage pattern, thus if something affects them than that’s bound to put effect on rotational cleavages in human embryos.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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