Authors
Takahito Yamasaki, Takehide Murata, Chunyuan Jin, Kohsuke Kato, Michiya Noguchi, Koji Nakade, Jianzhi Pan, Kyousuke Nagata, and Kazunari Yokoyama
Summary
At present, the best-defined and most useful source of material for reconstitution of chromatin consists of purified core histones. These core histones can be prepared from either solubilized chromatin or H1-depleted chromatin by column chromatography on hydroxyapatite. To maintain the correct stoichiometry of all histones, we recommend preparation from H1-depleted chromatin by single-step elution with 2 M NaCl (see Note 1). For a detailed introduction to assays of nucleosome assembly and the inhibition of histone acetyltransferase activity, please go here: http://www.natureprotocols.com/2007/07/30/assays_of_nucleosome_assembly.phpFurther details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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