Authors
Takahito Yamasaki, Takehide Murata, Chunyuan Jin, Kohsuke Kato, Michiya Noguchi, Koji Nakade, Jianzhi Pan, Kyousuke Nagata, and Kazunari Yokoyama
Summary
The most efficient method for solubilizing the chromatin in isolated nuclei is to digest nuclei with Micrococcal nuclease (see Note 1). Linker histones are then removed from the solubilized chromatin by FPLC on a hydroxyapatite column (protocol A) or by a batch method with a cation-exchange resin (protocol B). For a detailed introduction to assays of nucleosome assembly and the inhibition of histone acetyltransferase activity, please go here: http://www.natureprotocols.com/2007/07/30/assays_of_nucleosome_assembly.phpFurther details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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