Authors
Takahito Yamasaki, Takehide Murata, Chunyuan Jin, Kohsuke Kato, Michiya Noguchi, Koji Nakade, Jianzhi Pan, Kyousuke Nagata, and Kazunari Yokoyama
Summary
We describe the protocol here using transcription factor JDP2 as a model protein with HAT-inhibitory and nucleosome-assembly activity. Filter-binding assays are performed as described elsewhere1 with minor modifications (see Figure 1a). For a detailed introduction to assays of nucleosome assembly and the inhibition of histone acetyltransferase activity, please go here: http://www.natureprotocols.com/2007/07/30/assays_of_nucleosome_assembly.phpFurther details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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