Authors
Junya Mitoma, Xingfeng Bao, and Minoru Fukuda
Summary
To analyze glycan function, enzyme digestion is one of the easiest method. We successfully demonstrated the elimination of N-glycans, O-glycans and heparan sulfate on lymph node frozen section by N-glycanase (N-glycosidase F), O-sialoglycopeptidase, and heparitinases, respectively. N-glycanase treatment usually needs denaturing condition such as SDS treatment, but this can be substituted with acetone treatment of the frozen section.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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