Authors
Andreas Hennig, Hüseyin Bakirci, and Werner M. Nau
Summary
Amino acid decarboxylases, which have been previously assayed with laborious manometric, radiometric or multi-step colorimetric methods, can be assayed by the simple addition of a commercial macrocycle (cucurbit-7-uril) and a fluorescent dye (Dapoxyl) to the enzymatic mixture. The protocol refers to lysine decarboxylase, but the activity of several other amino acid decarboxylases can be similarly determined.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 251
- Comments 0