Authors
Jeff Roberts
Summary
Animal models of the early events of papillomavirus (PV) infection have generally used animal PV types and have examined the skin or the oral mucosa as sites of infection. We have developed a mouse model for human sexual transmission by utilizing genital-tropic human papillomavirus (HPV) types to infect the cervicovaginal epithelium. Under specific conditions, human papillomavirus (HPV) vectors – viral capsids encapsidating a reporter plasmid in place of the authentic PV genome, known as pseudoviruses (PsV) – will transduce the genital epithelium, recapitulating the establishment phase of infection. Application of the genital challenge model is described here, with particular attention to the methods for inducing susceptibility to infection through mechanical or chemical disruption of the genital mucosa and to the technique and instruments required to produce infection at the squamocolumnar junction located in the endocervical canal.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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