Authors
Mohit Prasad, Anna C-C Jang, and Denise J. Montell
Summary
Drosophila oogenesis serves as a model for studies of a wide variety of biological problems, including stem cell biology, pattern formation, actin cytoskeleton dynamics, cell polarity, ad cell migration. We and others have studied border cells in the Drosophila ovary as a genetically-tractable example of cell migration in vivo 1-10). Border cells migrate as coherent cluster of 6-10 cells and are specified at the anterior tip of the early stage 8 egg chamber11. Over the years a number of mutants have been isolated and characterized that exhibit defective border cell migration, and many genes have been shown to control different aspects of border cell migration4,5. However a significant limitation has been that it has not been possible to observe their migration live. We recently reported the development of culture conditions that support border cell migration and egg chamber development from stage 8 to stage 10 (ref. 10). Here we describe in detail how to carry out live imaging of stage 9 egg chambers. The conditions we have defined also permit growth and development of the entire egg chamber, enabling live studies of other aspects of oogenesis, including outer follicle cell epithelial rearrangement and oocyte growth.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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