Authors
Regina Krohn, Christian Weber, Rory R. Koenen, and Juergen Bernhagen
Summary
In our study, we have discovered chemokine-like functions for the cytokine MIF such as induction of leukocyte arrest on endothelial cells. Cell adhesion requires the activation, i.e. by arrest chemokines, of integrins on the cell surface, which subsequently interact with adhesion molecules, such as VCAM-1 and ICAM-1. The latter interacts with the alpha-L beta-2 integrin. As MIF, like CXCL8, induced monocyte arrest on ICAM-1-expressing CHO cells1, we investigated the direct effect of MIF on integrin activation.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
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