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Identification of target mRNAs of regulatory RNA binding proteins using mRNP immunopurification and microarrays

External protocol Created on 03 May 2014

Authors

Mayka Sanchez, Bruno Galy, Matthias W. Hentze, and Martina U. Muckenthaler

Summary

RNA-binding proteins (RBPs) frequently regulate the posttranscriptional fate of target mRNAs. To identify novel target mRNAs of RBPs, we established a protocol for the immunoprecipitation of ribonucleoprotein complexes and the identification of associated mRNAs by microarray analysis.

Further details

The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.

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