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EphB2-Fc precipitates from rat hippocampal neurons in culture and from brain lysates

External protocol Created on 03 May 2014

Authors

Inmaculada Segura, Clara L. Essmann, Stefan Weinges, and Amparo Acker-Palmer

Summary

EphrinBs are transmembrane ligands for the Eph receptor tyrosine kinases. Here we describe the procedure used to precipitate ephrinB signaling complexes from hippocampal neurons in culture or brain lysates using soluble EphB receptor ectodomains fused to Fc portions of human IgG (EphB2-Fc).

Further details

The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.

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