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SLIC sub-cloning using T4 DNA polymerase treated inserts without RecA

External protocol Created on 03 May 2014

Authors

Mamie Z. Li and Stephen J. Elledge

Summary

We describe a novel cloning method SLIC that allows the assembly of multiple DNA fragments in a single reaction using in vitro homologous recombination and single-strand annealing.

Further details

The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.

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