Authors
Yuka Okada and Masahito Ikawa
Summary
Conventional transgenic approaches manipulate both fetal and placental genome, so that it is not clear if symptoms originate from placental and/or fetal effects. Moreover, germ-line transmission of a transgene is obstacle for therapeutic application. Here we detail a placenta specific gene manipulation by transducing blastocysts with lentiviral vectors1. After a removal of zona pellucida which functions as a physical barrier, trophoblast cells lying outermost layer of blastocyst were transduced from outside with high-titer lentiviral vectors. As most placental cells descend from trophoblast cells while fetus originated from inner cell mass, transgene expression can be observed in trophoblast cells from preimplantation stages and in placenta throughout gestation. Transgenic placentas can be generated at 100% of efficiency while all fetuses remain non-transgenic. This technology provides a robust system for studying placental organogenesis with implications for the treatment of placental dysfunction.Further details
The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 301
- Comments 0