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Directed yeast two hybrid screening for MEKK1 interaction partners

External protocol Created on 03 May 2014

Authors

Ewen Gallagher, Thomas Enzler, Atsushi Matsuzawa, and Michael Karin

Summary

This protocol utilizes the Y190 yeast strain, that characteristically turns from white to red during growth due to ade- mutation. Baits were subcloned into the pAS1cyh2 vector, and Preys subcloned into pACT2 (5).

Further details

The protocol was published on Protocol Exchange in 2007. To see the entire protocol, click on the source link.

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