Authors
Markus Hafner, Anton Schmitz, and Michael Famulok
Summary
SecinH3 causes insulin resistance in mouse liver and in cell culture by inhibiting cytohesins that are effectors of the insulin signalling pathway. Stimulation with insulin leads to increased transcription of glycolytic genes, to suppressed transcription of gluconeogenetic genes and IGFBP1 in liver and in HepG2 cells. These insulin effects can be reverted by SecinH3. We quantified gene expression levels by real-time PCR.Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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