Authors
Liming He, Xin-Sheng Wu, Raja Mohan, and Ling-Gang Wu
Summary
The cell-attached capacitance recording technique is a powerful technique that has been successfully used to resolve single vesicle fusion and fusion pore conductance1. This technique, however, has not been applied to synapses because of the difficulty in accessing release sites. Here, we developed a technique to expose release sites in a large nerve terminal, the calyx of Held, which contains clear-core glutamatergic vesicles. At the exposed presynaptic release sites, we performed low noise cell-attached capacitance recordings, which allowed us to resolve single vesicle fusion and fusion pore conductance.Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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