Authors
Charles N. Serhan, Amiram Ariel, Gabrielle Fredman, Yee-Ping Sun, Alpdogan Kantarci, Thomas E. Van Dyke, and Andrew D. Luster
Summary
During the resolution phase of inflammation the elimination of chemokines from the inflammatory milieu is accompanied by nonphlogistic clearance of corpses of apoptotic leukocytes by macrophages. Here, a method that determines the role of these apoptotic leukocytes in the termination of pro-inflammatory chemokines, like CCL3 and CCL5 was established. The zymosan A-initiated murine peritonitis, a self resolving sterile inflammation model, was utilized and sorted apoptotic neutrophils from Ccr5 minus/minus or wild type mice to Ccr5 minus/minus recipients were transferred. Following a scavenging period the peritoneal exudates were recovered, and it was determined that the levels of CCR5 ligands in the exudates of Ccr5 minus/minus recipients were increased over the levels of these mediators in wild type recipients. Application of lipid extraction from peritoneal exudates, in tandem with lipid mediator informatics can be used to determine the role of apoptotic neutrophils in the generation of resolution phase lipid mediators. This neutrophil transfer system allows the determination of the direct impact of apoptotic leukocytes in the resolution of inflammation.Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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