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Bead aggregation assay to demonstrate the clustering of postsynaptic proteins by the NGL family of cell adhesion molecules

External protocol Created on 03 May 2014

Authors

Seho Kim and Eunjoon Kim

Summary

Bead-induced direct aggregation of neuroligin on the surface membrane of neuronal dendrites induced the clustering of neuroligin and various postsynaptic proteins [1]. We employed this assay to demonstrate that direct aggregation of NGL, a family of cell adhesion molecules that associates with the netrin-G family of cell adhesion molecules and the postsynaptic scaffolding protein PSD-95, on the dendritic surface induces coclustering of excitatory postsynaptic proteins including PSD-95, GKAP, Shank, and NMDA glutamate receptors.

Further details

The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.

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