Authors
Dong-Er Zhang, Ming Yan, and Anita Boyapati
Summary
Diverse transcripts are generated by chromosomal translocation 8;21 (1-6). We reported that one previously unidentified and alternatively spliced transcript, AML1-ETO including ETO exon 9a (AE9a) (Figure 1, ref. 1), is leukemogenic in mice and is widely detected in t(8;21) AML patients (7). Here, we describe the methods to identify and quantitate the specific A/E9a transcript in t(8;21) patients samples relative to the AML1-ETO transcript encoding the well known full-length 752 amino acid AML1-ETO protein (AE).Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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