Authors
Irina Naguibneva
Summary
Four copies of miR-181a target site from mouse HoxA-11gene have been cloned between SacI and XbaI sites of pISO vector (kind gift of David Bartel), containing a MCS downstream of Firefly Luciferase ORF. The cloning strategy used compatible cohesive ends SpeI/XbaI to enter sequentially 1,2,4 etc. copies of target sequences (Figure 1).Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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