Authors
Hideyo Hirai, Pu Zhang, Tajhal Dayaram, Christopher Hetherington, Shin-ichi Mizuno, Jiro Imanishi, Koichi Akashi, and Daniel Tenen
Summary
The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. Here, we described our standard procedures of the murine myeloid progenitor staining for fluorescence activated cells sorting (FACS) and RNA purification methods.Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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