Authors
Hideyo Hirai, Pu Zhang, Tajhal Dayaram, Christopher Hetherington, Shin-ichi Mizuno, Jiro Imanishi, Koichi Akashi, and Daniel Tenen
Summary
This highly efficient in vivo gene transduction technique for laboratory mice was performed basically as described previously (Gene Therapy. 1999 Jul; 6(7):1258-1266). Hepatocytes are most effectively transduced by tail vein injection of a large volume of DNA solution in a short time. Practice with the injection technique is necessary!Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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