Authors
Anelia Horvath, Sosipatros Boikos, Kevin Cramer, and Constantine Stratakis
Summary
Genome wide genotyping was ran on Affymetrix 10K xba 142 arrays producing 10,000 plus genotypes for each sample. The samples themselves were derived from the adrenal tissue of affected individuals as well as from peripheral blood. There were 35 samples in total with 18 affected and 17 unaffected. Two samples existed for most cases with one from peripheral blood and the other directly from the tumor. When possible, blood samples were collected from unaffected parents and these served as the controls in the study. There is some ethnic diversity within the group, although most were Caucasian. The cases phenotype was a form of adrenal cancer called “Micronodular (non-pigmented) Adrenocortical Hyperplasia” (MAH) that occurs early in life. Exemplar Genotyping Analysis Suite was applied to perform various analyses on the supplied data. The objective was to reduce the 10,000+ SNP’s to a smaller subset of interesting candidates for further exploration.Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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