Authors
Bakhos A. Tannous, Jan Grimm, Katherine Perry, Ralph Weissleder, and Xandra O. Breakefield
Summary
A versatile technique for labeling and imaging cells in culture and in vivo is presented. A metabolically biotinylated reporter protein is expressed on the cell surface which can then be tracked with any imaging agent coupled to streptavidin. A model recombinant reporter protein incorporates the prokaryotic Propionibacterium shermanii 1.3S transcarboxylase domain biotin acceptor peptide (BAP) between an N-terminal signal sequence and the platelet derived growth factor receptor transmembrane domain (TM)1. The BAP sequence provides a means to track any protein and the BAP-TM reporter allows for non-invasive real time imaging of any cell type transduced to express it in culture or in vivo using a variety of techniques including optical1,2, magnetic resonance1,3 and positron emission tomogrophic imaging4.Further details
The protocol was published on Protocol Exchange in 2006. To see the entire protocol, click on the source link.Advertisement
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