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Cell Lysis Buffers or Protein Lysis Buffers

Recipe Created on 07 Jan 2016

Procedure

NP-40 Lysis buffer:

140mM  NaCl
1.0% NP-40
50 mM Tris-HCl pH 8.0

 

High Salt Lysis Buffer:

500 mM NaCl
1.0% NP-40
50 mM Tris-HCl pH 8.0

 

Low Salt Lysis Buffer:

1.0% NP-40
50 mM Tris-HCl pH 8.0

 

RIPA Buffer:

150 mM NaCl
1% NP-40
0.5% NaDeoxycholate
0.1% SDS
50 mM Tris-HCl pH 8.0

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