Summary
Erythrocytes Lysing Buffer
Materials
| Reagent | Quantity(FOR 1L) | Final concentration |
| NH4Cl | 8.26 g | 150 mM |
| KHCO3 | 1 g | 10 mM |
| Na2EDTA | 37.2 mg | 0.1 mM |
Procedure
Dissolve all reagents in 850 mL of H2O. Adjust the pH to 7.2–7.4, and add H2O to 1000 mL. Store for up to 6 mo at room temperature.
References
doi:10.1101/pdb.rec083295
Cold Spring Harb Protoc 2014.
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