Authors
Alina Rodríguez-Mallon, Yamilet Cárdenas, Juana María Lugo, Aymé Oliva, Antonio Morales, Mario Pablo Estrada
Summary
Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is designed and validated. This experimental procedure was used to determine the abundance of growth hormone receptor type I transcript in different tilapia tissues. The results obtained with this developed competitive RT-PCR were similar to real-time PCR results reported recently. This protocol provides a reliable alternative, but less expensive than real-time PCR to quantify specific genes.Further details
The protocol was imported from Springer Protocols. To see the entire protocol, click on the source link, or download the pdf here.Advertisement
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