Authors
Creative Bioarray
Summary
Patch-clamp, albeit technically difficult, remains the most reliable and resolutive method to study ionic channels. Manual patch-clamp is the "gold-standard" for the investigation of ion channel activity. It can be applied in:
cell lines
primary and stem cell-derived cells
tissue slices
Acroscell offers routine assays to study drug effects on voltage-gated and ligand-gated ion channels as well as customer-tailored solutions and assay development.
Introduction
Manual patch-clamp is a versatile, high-resolution and low-throughput method which measures the currents generated by a single cell upon stimulation. Thanks to the flexibility of the patch-clamp technique, virtually all ionic channels in the body: ligand-gated, voltage-gated, calcium-gated, fast/slow activating and deactivating - can be studied with unmatched resolution using well-designed electrophysiology protocols.
In addition to confirming the activity of potential hits from high or medium throughput screens, manual patch-clamping can be used to assess mechanism of action of compounds and to determine the effects of compounds on the biophysical properties of a channel. Manual patch-clamp can be used for safety evaluation in drug development and lead optimization (eg, manual patch clamp of the hERG potassium channel to evaluate potential cardiac liability).
With manual patch-clamp, very precise physiological and pharmacological questions could be addressed for understanding of mechanisms of action. Both voltage-gated and ligand-gated channels can be tested using manual patch-clamping. This system utilizes stable cell lines or native cells (neurons, cardiac myocytes, etc.). It is the most accurate way of analyzing ion channel activities.
In neuroscience research, manual patch-clamp technology provides the highest resolution for electrophysiological recordings from single neuron down to single channel thanks to the different recording configurations (whole-cell, perforated-patch, cell-attached, outside-out).
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