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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

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External protocol03 May 2014

Isolation of Treg cells and Treg cell suppression/death assay

by Pushpa Pandiyan
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External protocol30 Apr 2014

Accurate measurement of cytosolic free zinc using eCALWY variants: a toolbox of genetically encoded FRET sensors

by Jan L. Vinkenborg, Elisa A. Bellomo, Tamara J. Nicolson, Melissa S. Koay, Maarten Merkx, and Guy A. Rutter
- Views 281
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External protocol30 Apr 2014

Purification of extracellular and intracellular amastigotes of Trypanosoma cruzi from mammalian host-infected cells

by Alexandre Marques, Ernesto Nakayasu, and Igor Almeida
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External protocol30 Apr 2014

A highly-sensitive multisubstrate-compatible chemiluminescent immunoassay for human fetuin A

by Sandeep Kumar Vashist
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External protocol10 Apr 2014

Fluorescence resonance energy transfer (FRET) as a method to calculate the dimerization strength of basic Helix-Loop-Helix (bHLH) proteins

by Victoria E. Centonze, Beth A. Firulli, Anthony B. Firulli
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External protocol10 Apr 2014

Expression, purification and characterization of ricin vectors used for exogenous antigen delivery into the MHC class I presentation pathway

by Daniel C. Smith, Catherine J. Marsden, J. Michael Lord, Lynne M. Roberts
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External protocol30 Apr 2014

A method for labeling polyacrylamide gels

by Arefeh Seyedarabi
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External protocol10 Apr 2014

A screen to identify drug resistant variants to target-directed anti-cancer agents

by Mohammad Azam, Tal Raz, Valentina Nardi, Sarah L. Opitz, George Q. Daley
- Views 280
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External protocol03 May 2014

Cell-attached capacitance measurements at release sites of a central nerve terminal

by Liming He, Xin-Sheng Wu, Raja Mohan, and Ling-Gang Wu
- Views 280
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External protocol30 Apr 2014

Enzyme-linked immunosorbent assay procedure at higher temperature enhances speed of the assay

by Pradip Nahar
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