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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

You can upload a protocol here

External protocol10 Apr 2014

Fluorescence resonance energy transfer (FRET) as a method to calculate the dimerization strength of basic Helix-Loop-Helix (bHLH) proteins

by Victoria E. Centonze, Beth A. Firulli, Anthony B. Firulli
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External protocol30 Apr 2014

Production of recombinant hBD-1 in Escherichia coli and its specific polyclonal antibody in rabbits

by Zhihong Wu, Bjoern O. Schroeder, Jens-M Schroeder, and Jan Wehkamp
- Views 283
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External protocol03 May 2014

Isolation of Treg cells and Treg cell suppression/death assay

by Pushpa Pandiyan
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External protocol10 Apr 2014

Promoter analysis by saturation mutagenesis

by Nitin S. Baliga
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External protocol03 May 2014

Assays of nucleosome assembly and the inhibition of histone acetyltransferase activity. (13) Ligation-mediated single-sided PCR (LMPCR)

by Takahito Yamasaki, Takehide Murata, Chunyuan Jin, Kohsuke Kato, Michiya Noguchi, Koji Nakade, Jianzhi Pan, Kyousuke Nagata, and Kazunari Yokoyama
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External protocol30 Apr 2014

ACEMBLing multigene expression vectors by recombineering

by Yan Nie, Christoph Bieniossek, Daniel Frey, Natacha Olieric, Christiane Schaffitzel, Michel O. Steinmetz, and Imre Berger
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External protocol10 Apr 2014

Expression, purification and characterization of ricin vectors used for exogenous antigen delivery into the MHC class I presentation pathway

by Daniel C. Smith, Catherine J. Marsden, J. Michael Lord, Lynne M. Roberts
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External protocol30 Apr 2014

A method for labeling polyacrylamide gels

by Arefeh Seyedarabi
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External protocol30 Apr 2014

Patch-Clamp Recording from Kenyon Cells in Acutely-Isolated Bee Brain

by Mary Palmer and Chris Connolly
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External protocol09 Apr 2014

A rapid and sensitive assay for quantification of siRNA efficiency and specificity

by Nicola Smart, Peter James Scambler, Paul Richard Riley
- Views 282
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