Authors
Sicheng Li, Haozheng Li, Yiran Li, Qi Zhang, Shuai Wang, Xin Lv, Shuai Yan, Zhiliang Huang, Xingbo Liu, Qipei Zhou, Bi Zhang, Long Xiao, Yage Chen, Zhe Wang, Wanjun Lu, Aiguo Shen, Jianfeng Liu, Ping Wang
Published in
Nature communications. Volume 16. Issue 1. Pages 3808. Apr 23, 2025. Epub Apr 23, 2025.
Abstract
Raman spectroscopy, which probes fine molecular vibrations, is crucial for interpreting covalent bonds, chemical compositions, and other molecular dynamics in mixtures via their vibrational fingerprint signatures. However, over the past few decades, longstanding barriers have been encountered in both the sensitivity and speed of Raman spectroscopy, limiting its ability to be extended to broader biochemical applications. Here, we introduce a versatile analytical workhorse, the fiber-array Raman engine (termed FIRE). In FIRE, a distinctive fiber array bundle delays the Raman shifts at a scale of 3-960 ns, and a highly dynamic single-channel photon-counting detector achieves spectral measurements that outperform the best commercial confocal Raman microscope. Crucially, FIRE features a major advantage of nonrepetitive single-shot spectra measurement at a MHz repetition rate with a full Raman span (-300-4300 cm-1) covering the fingerprint, silent, C-H, and O-H regions and therefore represents a major step toward overall improving of sensitivity, speed, and spectral span. We demonstrate full Raman spectral imaging of the metabolic activity of intact Caenorhabditis elegans. FIRE exhibits superior performance to a Raman microscope in all aspects, including autofluorescence suppression, and will elucidate a variety of biochemical applications.
PMID:
40268912
Bibliographic data and abstract were imported from PubMed on 24 Apr 2025.
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