Authors
Pan Wang, Jixiang Zhang, Minghui Zhan, Zicong Tan, Chao Wang, Bianhua Liu, Zhenyang Wang, Jun Zhao
Published in
ACS applied bio materials. Apr 28, 2025. Epub Apr 28, 2025.
Abstract
The current vitrification-based oocyte cryopreservation relies on inherently cytotoxic high-concentration cryoprotective agents (CPAs), leading to suboptimal post-thaw survival rates and diminished developmental competence. To address this, we developed a series of highly biocompatible amino-functionalized iron-based metal-organic frameworks (Fe-MOFs) via a facile hydrothermal method to synergistically suppress ice crystallization and minimize the reliance on toxic CPAs. Among the synthesized materials, 2NH2-MIL88(Fe) demonstrated exceptional ice-inhibition performance, reducing ice crystal size to 16.78% of that observed in pure water, significantly outperforming its nonaminated counterpart MIL88(Fe) and monoaminated counterpart NH2-MIL88(Fe). Notably, 2NH2-MIL88(Fe) exhibited an unprecedented intrinsic photothermal response without requiring photosensitizer modification, achieving rapid temperature elevation from 25 to 86.6 °C under 808 nm laser irradiation (1 W/cm2, 1 min). When applied to mouse oocyte cryopreservation, this dual-functional amino-MOF enabled complete elimination of dimethyl sulfoxide (DMSO) from CPAs while maintaining an exceptional post-thaw survival rate of 95.1%. Our findings establish a dual correlation between amino group density and both ice-growth suppression efficiency and photothermal performance, revealing a synergistic mechanism for mouse oocyte cryopreservation enhancement. This structure-function relationship provides critical insights for the rational design of next-generation nano-CPAs.
PMID:
40294340
Bibliographic data and abstract were imported from PubMed on 29 Apr 2025.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 35
- Comments 0